Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo

Post Date: 
2005-07-15
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Publication: 
AIDS Research and Human Retroviruses
Summary: 
As the HIV-1 pandemic becomes increasingly complex, the genetic characterization of HIV strains bears important implications for vaccine research. To better understand the molecular evolution of HIV-1 viral diversity, we performed a comparative molecular analysis of HIV strains collected from high-risk persons in Kinshasa, Democratic Republic of Congo (DRC). Analysis of the gag-p24, env-C2V3 and -gp41 regions from 83 specimens collected in 1999-2000 revealed that 44 (53%) had concordant subtypes in the three regions (14 subsubtype A1, 10 subtype G, 8 subtype D, 5 subtype C, 2 each subsubtype F1 and CRF01_AE, and one each of subtypes H and J, and subsubtype A2, while the remaining 39 (47%) had mosaic genomes comprising multiple subtype combinations. Similar multisubtype patterns were also observed in 24 specimens collected in 1985. Sequence analysis of the gag-pol region (2.1 kb) from 21 discordant specimens in the gag-p24, env-C2V3 and -gp41 regions in 1985 and 1999-2000 further confirmed the complex recombinant patterns. Despite the remarkable similarity in overall subtype distribution, the intra- and intersubtype distances of major subtypes A1 and G increased significantly from 1985 to 1999-2000 (p=0.018 and p=0.0016, respectively). Given the complexity of HIV-1 viruses circulating in DRC, efforts should focus on the development of vaccines that result in cross-clade immunity.
Citation: 
Yang C, Ming L, Mokili JLK, Winter J, Lubaki NM, Kashamuka MN, Kasali MJ, Losoma AJ, Quinn TC, Bollinger RC, Lal RB. Genetic diversification and recombination of HIV type 1 group M in Kinshasa, Democratic Republic of Congo. AIDS Res Hum Retroviruses. 2005 Aug 1; 21(7):661-666.
Collaborators: 
  • Centers for Disease Control and Prevention, Atlanta, GA
  • Los Alamos National Laboratory, Los Alamos, New Mexico
  • Programme National de Lutte contre le SIDA, Kinshasa, Democratic Republic of Congo